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affinity purified polyclonal antibody against elac2  (Proteintech)


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    Proteintech affinity purified polyclonal antibody against elac2
    (A) Sub-organellar localization of <t>Elac2</t> by western-blot analysis on mitochondria isolated from HeLa cells. Specificity of antibody reactivity was proved by the CRM obtained using an in vitro translated (i.v.) Elac2. A CRM was present in mitochondria (M), in the mitochondrial matrix (Ma) and membranes (Mb), and barely detectable in the nucleus (N). ETHE1 antibody was used as a mitochondrial matrix control protein. (B) Proteinase K protection assay by western-blot analysis on freshly isolated mitochondria from HeLa cells. Lane 1, cell lysate; lane 2, intact mitochondria; lane 3, mitochondria treated with 100 µg/ml of proteinase K; lane 4, mitochondria treated with 0.5% of Triton-X100. (C) Mitochondrial in vivo import of HA-tagged human Elac2 N-terminal polypeptide expressed in Cos-7 cells. Lane 1: marker 46 kDa (MW). lane 2: immunoprecipitation of total radiolabeled proteins. lane 3: immunoprecipitation of total radiolabeled proteins after the addition of valinomycin that determines the elimination of the mitochondrial ΔΨ: as a consequence, mitochondrial import is abolished. lane 4: immunoprecipitation of in vitro translation product (i.v.). Arrows indicate the Elac2 precursor (Elac2) and the mature form (mElac2). (D) Confocal immunofluorescence on Cos7 cells. The green fluorescence corresponds to Elac2HA-specific immunoreactivity. The red fluorescence corresponds to immunoreactivity specific to mtSSB. The two immunofluorescence patterns overlap, as shown by confocal merge. Magnification: 40×.
    Affinity Purified Polyclonal Antibody Against Elac2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified polyclonal antibody against elac2/product/Proteintech
    Average 93 stars, based on 9 article reviews
    affinity purified polyclonal antibody against elac2 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "How Do Human Cells React to the Absence of Mitochondrial DNA?"

    Article Title: How Do Human Cells React to the Absence of Mitochondrial DNA?

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0005713

    (A) Sub-organellar localization of Elac2 by western-blot analysis on mitochondria isolated from HeLa cells. Specificity of antibody reactivity was proved by the CRM obtained using an in vitro translated (i.v.) Elac2. A CRM was present in mitochondria (M), in the mitochondrial matrix (Ma) and membranes (Mb), and barely detectable in the nucleus (N). ETHE1 antibody was used as a mitochondrial matrix control protein. (B) Proteinase K protection assay by western-blot analysis on freshly isolated mitochondria from HeLa cells. Lane 1, cell lysate; lane 2, intact mitochondria; lane 3, mitochondria treated with 100 µg/ml of proteinase K; lane 4, mitochondria treated with 0.5% of Triton-X100. (C) Mitochondrial in vivo import of HA-tagged human Elac2 N-terminal polypeptide expressed in Cos-7 cells. Lane 1: marker 46 kDa (MW). lane 2: immunoprecipitation of total radiolabeled proteins. lane 3: immunoprecipitation of total radiolabeled proteins after the addition of valinomycin that determines the elimination of the mitochondrial ΔΨ: as a consequence, mitochondrial import is abolished. lane 4: immunoprecipitation of in vitro translation product (i.v.). Arrows indicate the Elac2 precursor (Elac2) and the mature form (mElac2). (D) Confocal immunofluorescence on Cos7 cells. The green fluorescence corresponds to Elac2HA-specific immunoreactivity. The red fluorescence corresponds to immunoreactivity specific to mtSSB. The two immunofluorescence patterns overlap, as shown by confocal merge. Magnification: 40×.
    Figure Legend Snippet: (A) Sub-organellar localization of Elac2 by western-blot analysis on mitochondria isolated from HeLa cells. Specificity of antibody reactivity was proved by the CRM obtained using an in vitro translated (i.v.) Elac2. A CRM was present in mitochondria (M), in the mitochondrial matrix (Ma) and membranes (Mb), and barely detectable in the nucleus (N). ETHE1 antibody was used as a mitochondrial matrix control protein. (B) Proteinase K protection assay by western-blot analysis on freshly isolated mitochondria from HeLa cells. Lane 1, cell lysate; lane 2, intact mitochondria; lane 3, mitochondria treated with 100 µg/ml of proteinase K; lane 4, mitochondria treated with 0.5% of Triton-X100. (C) Mitochondrial in vivo import of HA-tagged human Elac2 N-terminal polypeptide expressed in Cos-7 cells. Lane 1: marker 46 kDa (MW). lane 2: immunoprecipitation of total radiolabeled proteins. lane 3: immunoprecipitation of total radiolabeled proteins after the addition of valinomycin that determines the elimination of the mitochondrial ΔΨ: as a consequence, mitochondrial import is abolished. lane 4: immunoprecipitation of in vitro translation product (i.v.). Arrows indicate the Elac2 precursor (Elac2) and the mature form (mElac2). (D) Confocal immunofluorescence on Cos7 cells. The green fluorescence corresponds to Elac2HA-specific immunoreactivity. The red fluorescence corresponds to immunoreactivity specific to mtSSB. The two immunofluorescence patterns overlap, as shown by confocal merge. Magnification: 40×.

    Techniques Used: Western Blot, Isolation, In Vitro, Control, In Vivo, Marker, Immunoprecipitation, Immunofluorescence, Fluorescence



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    affinity purified polyclonal antibody against elac2  (Proteintech)
    93
    Proteintech affinity purified polyclonal antibody against elac2
    (A) Sub-organellar localization of <t>Elac2</t> by western-blot analysis on mitochondria isolated from HeLa cells. Specificity of antibody reactivity was proved by the CRM obtained using an in vitro translated (i.v.) Elac2. A CRM was present in mitochondria (M), in the mitochondrial matrix (Ma) and membranes (Mb), and barely detectable in the nucleus (N). ETHE1 antibody was used as a mitochondrial matrix control protein. (B) Proteinase K protection assay by western-blot analysis on freshly isolated mitochondria from HeLa cells. Lane 1, cell lysate; lane 2, intact mitochondria; lane 3, mitochondria treated with 100 µg/ml of proteinase K; lane 4, mitochondria treated with 0.5% of Triton-X100. (C) Mitochondrial in vivo import of HA-tagged human Elac2 N-terminal polypeptide expressed in Cos-7 cells. Lane 1: marker 46 kDa (MW). lane 2: immunoprecipitation of total radiolabeled proteins. lane 3: immunoprecipitation of total radiolabeled proteins after the addition of valinomycin that determines the elimination of the mitochondrial ΔΨ: as a consequence, mitochondrial import is abolished. lane 4: immunoprecipitation of in vitro translation product (i.v.). Arrows indicate the Elac2 precursor (Elac2) and the mature form (mElac2). (D) Confocal immunofluorescence on Cos7 cells. The green fluorescence corresponds to Elac2HA-specific immunoreactivity. The red fluorescence corresponds to immunoreactivity specific to mtSSB. The two immunofluorescence patterns overlap, as shown by confocal merge. Magnification: 40×.
    Affinity Purified Polyclonal Antibody Against Elac2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified polyclonal antibody against elac2/product/Proteintech
    Average 93 stars, based on 1 article reviews
    affinity purified polyclonal antibody against elac2 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Sub-organellar localization of Elac2 by western-blot analysis on mitochondria isolated from HeLa cells. Specificity of antibody reactivity was proved by the CRM obtained using an in vitro translated (i.v.) Elac2. A CRM was present in mitochondria (M), in the mitochondrial matrix (Ma) and membranes (Mb), and barely detectable in the nucleus (N). ETHE1 antibody was used as a mitochondrial matrix control protein. (B) Proteinase K protection assay by western-blot analysis on freshly isolated mitochondria from HeLa cells. Lane 1, cell lysate; lane 2, intact mitochondria; lane 3, mitochondria treated with 100 µg/ml of proteinase K; lane 4, mitochondria treated with 0.5% of Triton-X100. (C) Mitochondrial in vivo import of HA-tagged human Elac2 N-terminal polypeptide expressed in Cos-7 cells. Lane 1: marker 46 kDa (MW). lane 2: immunoprecipitation of total radiolabeled proteins. lane 3: immunoprecipitation of total radiolabeled proteins after the addition of valinomycin that determines the elimination of the mitochondrial ΔΨ: as a consequence, mitochondrial import is abolished. lane 4: immunoprecipitation of in vitro translation product (i.v.). Arrows indicate the Elac2 precursor (Elac2) and the mature form (mElac2). (D) Confocal immunofluorescence on Cos7 cells. The green fluorescence corresponds to Elac2HA-specific immunoreactivity. The red fluorescence corresponds to immunoreactivity specific to mtSSB. The two immunofluorescence patterns overlap, as shown by confocal merge. Magnification: 40×.

    Journal: PLoS ONE

    Article Title: How Do Human Cells React to the Absence of Mitochondrial DNA?

    doi: 10.1371/journal.pone.0005713

    Figure Lengend Snippet: (A) Sub-organellar localization of Elac2 by western-blot analysis on mitochondria isolated from HeLa cells. Specificity of antibody reactivity was proved by the CRM obtained using an in vitro translated (i.v.) Elac2. A CRM was present in mitochondria (M), in the mitochondrial matrix (Ma) and membranes (Mb), and barely detectable in the nucleus (N). ETHE1 antibody was used as a mitochondrial matrix control protein. (B) Proteinase K protection assay by western-blot analysis on freshly isolated mitochondria from HeLa cells. Lane 1, cell lysate; lane 2, intact mitochondria; lane 3, mitochondria treated with 100 µg/ml of proteinase K; lane 4, mitochondria treated with 0.5% of Triton-X100. (C) Mitochondrial in vivo import of HA-tagged human Elac2 N-terminal polypeptide expressed in Cos-7 cells. Lane 1: marker 46 kDa (MW). lane 2: immunoprecipitation of total radiolabeled proteins. lane 3: immunoprecipitation of total radiolabeled proteins after the addition of valinomycin that determines the elimination of the mitochondrial ΔΨ: as a consequence, mitochondrial import is abolished. lane 4: immunoprecipitation of in vitro translation product (i.v.). Arrows indicate the Elac2 precursor (Elac2) and the mature form (mElac2). (D) Confocal immunofluorescence on Cos7 cells. The green fluorescence corresponds to Elac2HA-specific immunoreactivity. The red fluorescence corresponds to immunoreactivity specific to mtSSB. The two immunofluorescence patterns overlap, as shown by confocal merge. Magnification: 40×.

    Article Snippet: An affinity purified polyclonal antibody against ELAC2 was from ProteinTech, while the polyclonal antibody against Ethe1 was raised in rabbit by Neosystem .

    Techniques: Western Blot, Isolation, In Vitro, Control, In Vivo, Marker, Immunoprecipitation, Immunofluorescence, Fluorescence